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raw microarray data (.cel files  (Thermo Fisher)


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    Thermo Fisher raw microarray data (.cel files
    Gene expression dataset . A) The gene expression dataset analyzed comprises cell samples from different levels of myeloid differentiation process (stem/progenitor cells, precursors and terminally differentiated cells). The graph describes relationships between the cellular contexts analyzed within myeloid differentiation tree. For each cell type, the number of samples examined with independent <t>microarray</t> experiments is indicated in brackets. B) Dendrogram obtained by unsupervised hierarchical clustering on gene expression data matrix. Pearson correlation and average were used as similarity measure and linking method, respectively.
    Raw Microarray Data (.Cel Files, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+raw+files/pmc02045681-248-2-5
    Average 90 stars, based on 1 article reviews
    raw microarray data (.cel files - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Genomic expression during human myelopoiesis"

    Article Title: Genomic expression during human myelopoiesis

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-8-264

    Gene expression dataset . A) The gene expression dataset analyzed comprises cell samples from different levels of myeloid differentiation process (stem/progenitor cells, precursors and terminally differentiated cells). The graph describes relationships between the cellular contexts analyzed within myeloid differentiation tree. For each cell type, the number of samples examined with independent microarray experiments is indicated in brackets. B) Dendrogram obtained by unsupervised hierarchical clustering on gene expression data matrix. Pearson correlation and average were used as similarity measure and linking method, respectively.
    Figure Legend Snippet: Gene expression dataset . A) The gene expression dataset analyzed comprises cell samples from different levels of myeloid differentiation process (stem/progenitor cells, precursors and terminally differentiated cells). The graph describes relationships between the cellular contexts analyzed within myeloid differentiation tree. For each cell type, the number of samples examined with independent microarray experiments is indicated in brackets. B) Dendrogram obtained by unsupervised hierarchical clustering on gene expression data matrix. Pearson correlation and average were used as similarity measure and linking method, respectively.

    Techniques Used: Expressing, Microarray

    Related Articles

    Microarray:

    Article Title: Transcriptomic screening to identify hub genes and drug signatures for PCOS based on RNA-Seq data in granulosa cells.
    Article Snippet: Background: Polycystic ovary syndrome (PCOS) is one of the most incident reproductive diseases, and remains the main cause of female infertility.. Granulosa cells play a critical role in normal follicle development and steroid hormones synthesis.. In spite of extensive research, no sole medication has been approved by FDA to treat PCOS.

    Article Title: Novel biomarkers and interferon signature in secondary progressive multiple sclerosis.
    Article Snippet: Multiple sclerosis (MS) exhibits poor immune regulation and subnormal interferon (IFN-β) signaling.. Secondary Progressive MS displays waning exacerbations, relentless neurodegeneration, and diminished benefit of therapy.. We find dysregulated serum protein balance (Th1/Th2) and excessive gene expression in Relapsing-Remitting MS vs. healthy controls (8700 differentially-expressed genes, DEG) and intermediate levels in SPMS (3900 DEG).

    Article Title: Evaluation of the p53 pathway in polycystic ovarian syndrome pathogenesis and apoptosis enhancement in human granulosa cells through transcriptome data analysis
    Article Snippet: .. The raw CEL microarray files were read into the Affy package of affylmGUI, a graphical user interface for the analysis of Affymetrix microarray data using the Linear Modes for MicroArray data (Limma) package , for microarray analysis and read counts were employed as input for RNA-seq data. ..

    Article Title: Genome-Wide Survey of Cold Stress Regulated Alternative Splicing in Arabidopsis thaliana with Tiling Microarray
    Article Snippet: .. A gene expression index was build for stress treatments comparison in a manner reminiscent of the Hormonometer algorithm : Affymetrix ATH1 microarray CEL files (containing raw data) of stress related experiments were retrieved from GEO. .. They were imported into Partek GS (Partek, Inc.) , RMA normalized and then the fold-change (treatment/control) of the genes and the statistical significance (using one-way ANOVA test) of that change were calculated.

    Article Title: Evaluation of the p53 pathway in polycystic ovarian syndrome pathogenesis and apoptosis enhancement in human granulosa cells through transcriptome data analysis.
    Article Snippet: .. The raw CEL microarray files were read into the Affy package of affylmGUI, a graphical user interface for the analysis of Affymetrix microarray data using the Linear Modes for MicroArray data (Limma) package64, for microarray analysis and read counts were employed as input for RNA-seq data. ..

    Article Title: Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean
    Article Snippet: Microarray data were analyzed using Expressionist Pro software from Genedata Inc. .. Raw data in the form of .CEL files from the Affymetrix GeneChip were uploaded to the platform, and the robust microarray analysis (RMA) algorithm [ ] was used to condense and normalize all soybean probeset data with a median of ten thousand. ..

    RNA Sequencing:

    Article Title: Transcriptomic screening to identify hub genes and drug signatures for PCOS based on RNA-Seq data in granulosa cells.
    Article Snippet: Background: Polycystic ovary syndrome (PCOS) is one of the most incident reproductive diseases, and remains the main cause of female infertility.. Granulosa cells play a critical role in normal follicle development and steroid hormones synthesis.. In spite of extensive research, no sole medication has been approved by FDA to treat PCOS.

    Article Title: Evaluation of the p53 pathway in polycystic ovarian syndrome pathogenesis and apoptosis enhancement in human granulosa cells through transcriptome data analysis
    Article Snippet: .. The raw CEL microarray files were read into the Affy package of affylmGUI, a graphical user interface for the analysis of Affymetrix microarray data using the Linear Modes for MicroArray data (Limma) package , for microarray analysis and read counts were employed as input for RNA-seq data. ..

    Article Title: Evaluation of the p53 pathway in polycystic ovarian syndrome pathogenesis and apoptosis enhancement in human granulosa cells through transcriptome data analysis.
    Article Snippet: .. The raw CEL microarray files were read into the Affy package of affylmGUI, a graphical user interface for the analysis of Affymetrix microarray data using the Linear Modes for MicroArray data (Limma) package64, for microarray analysis and read counts were employed as input for RNA-seq data. ..

    other:

    Article Title: Influence of a Short-Term Iron-Deficient Diet on Hepatic Gene Expression Profiles in Rats
    Article Snippet: We quantified the raw microarray data (Affymetrix CEL files) using a robust multiarray average (RMA).

    Generated:

    Article Title: Novel biomarkers and interferon signature in secondary progressive multiple sclerosis.
    Article Snippet: Multiple sclerosis (MS) exhibits poor immune regulation and subnormal interferon (IFN-β) signaling.. Secondary Progressive MS displays waning exacerbations, relentless neurodegeneration, and diminished benefit of therapy.. We find dysregulated serum protein balance (Th1/Th2) and excessive gene expression in Relapsing-Remitting MS vs. healthy controls (8700 differentially-expressed genes, DEG) and intermediate levels in SPMS (3900 DEG).

    Software:

    Article Title: Novel biomarkers and interferon signature in secondary progressive multiple sclerosis.
    Article Snippet: Multiple sclerosis (MS) exhibits poor immune regulation and subnormal interferon (IFN-β) signaling.. Secondary Progressive MS displays waning exacerbations, relentless neurodegeneration, and diminished benefit of therapy.. We find dysregulated serum protein balance (Th1/Th2) and excessive gene expression in Relapsing-Remitting MS vs. healthy controls (8700 differentially-expressed genes, DEG) and intermediate levels in SPMS (3900 DEG).

    Gene Expression:

    Article Title: Genome-Wide Survey of Cold Stress Regulated Alternative Splicing in Arabidopsis thaliana with Tiling Microarray
    Article Snippet: .. A gene expression index was build for stress treatments comparison in a manner reminiscent of the Hormonometer algorithm : Affymetrix ATH1 microarray CEL files (containing raw data) of stress related experiments were retrieved from GEO. .. They were imported into Partek GS (Partek, Inc.) , RMA normalized and then the fold-change (treatment/control) of the genes and the statistical significance (using one-way ANOVA test) of that change were calculated.

    Comparison:

    Article Title: Genome-Wide Survey of Cold Stress Regulated Alternative Splicing in Arabidopsis thaliana with Tiling Microarray
    Article Snippet: .. A gene expression index was build for stress treatments comparison in a manner reminiscent of the Hormonometer algorithm : Affymetrix ATH1 microarray CEL files (containing raw data) of stress related experiments were retrieved from GEO. .. They were imported into Partek GS (Partek, Inc.) , RMA normalized and then the fold-change (treatment/control) of the genes and the statistical significance (using one-way ANOVA test) of that change were calculated.

    Analysis:

    Article Title: Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean
    Article Snippet: Microarray data were analyzed using Expressionist Pro software from Genedata Inc. .. Raw data in the form of .CEL files from the Affymetrix GeneChip were uploaded to the platform, and the robust microarray analysis (RMA) algorithm [ ] was used to condense and normalize all soybean probeset data with a median of ten thousand. ..



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    Image Search Results


    Gene expression dataset . A) The gene expression dataset analyzed comprises cell samples from different levels of myeloid differentiation process (stem/progenitor cells, precursors and terminally differentiated cells). The graph describes relationships between the cellular contexts analyzed within myeloid differentiation tree. For each cell type, the number of samples examined with independent microarray experiments is indicated in brackets. B) Dendrogram obtained by unsupervised hierarchical clustering on gene expression data matrix. Pearson correlation and average were used as similarity measure and linking method, respectively.

    Journal: BMC Genomics

    Article Title: Genomic expression during human myelopoiesis

    doi: 10.1186/1471-2164-8-264

    Figure Lengend Snippet: Gene expression dataset . A) The gene expression dataset analyzed comprises cell samples from different levels of myeloid differentiation process (stem/progenitor cells, precursors and terminally differentiated cells). The graph describes relationships between the cellular contexts analyzed within myeloid differentiation tree. For each cell type, the number of samples examined with independent microarray experiments is indicated in brackets. B) Dendrogram obtained by unsupervised hierarchical clustering on gene expression data matrix. Pearson correlation and average were used as similarity measure and linking method, respectively.

    Article Snippet: In particular, raw microarray data (Affymetrix .CEL files) were obtained for a total of 24 samples from 8 different cell types of all myelopoietic lineages, representing a reference dataset for a comprehensive analysis of genomic expression during cell differentiation.

    Techniques: Expressing, Microarray

    Genetic deletion of SphK1 reduced CLDN2 in HER2/neu-induced mice breast tumors. (A) Whole genome expression profile evaluated by microarray analysis assessed the expression of 39000 gene transcripts. Gene expression microarray heatmap (n = 2 per group) of dysregulated genes. (B) Twenty molecules with the most significant expression changes (10 up-regulation and 20 down-regulation) between SphK1+/+ and SphK1−/− tumors are shown. (C) CLDN2 mRNA expression in tumors from SphK1+/+ and SphK1−/− mice was confirmed by qRT-PCR analysis and analyzed by unpaired t-test. ****P ≤ 0.0001 vs. SphK1+/+. (D) Representative paraffin-tumor sections immunostained for CLDN2 in tumors from SphK1+/+ and SphK1−/− mice (P = 0.1339).

    Journal: Carcinogenesis

    Article Title: Genetic deletion of sphingosine kinase 1 suppresses mouse breast tumor development in an HER2 transgenic model

    doi: 10.1093/carcin/bgx097

    Figure Lengend Snippet: Genetic deletion of SphK1 reduced CLDN2 in HER2/neu-induced mice breast tumors. (A) Whole genome expression profile evaluated by microarray analysis assessed the expression of 39000 gene transcripts. Gene expression microarray heatmap (n = 2 per group) of dysregulated genes. (B) Twenty molecules with the most significant expression changes (10 up-regulation and 20 down-regulation) between SphK1+/+ and SphK1−/− tumors are shown. (C) CLDN2 mRNA expression in tumors from SphK1+/+ and SphK1−/− mice was confirmed by qRT-PCR analysis and analyzed by unpaired t-test. ****P ≤ 0.0001 vs. SphK1+/+. (D) Representative paraffin-tumor sections immunostained for CLDN2 in tumors from SphK1+/+ and SphK1−/− mice (P = 0.1339).

    Article Snippet: Partek pre-processes raw intensity files from microarray experiment using GCRMA’s background subtraction and uses quantile normalization as the normalization technique.

    Techniques: Expressing, Microarray, Gene Expression, Quantitative RT-PCR

    SphK1 and CLDN2 levels are increased in HER2-positive breast cancers in humans. Tissue microarray containing breast cancer samples from 92 HER2-positive breast cancer patients and matched adjacent normal breast tissues (n = 34) are stained for SphK1 and CLDN2. Staining were scored based on the intensity (0–3) and proportion (0–3), and the total scores were used for the analysis. [(A) and (B)] SphK1 and CLDN2 expressions were compared with pathological features including grade, stage and hormone receptor status. [(C) and (D)] Representative image of high, moderate and low SphK1 and CLDN2 expressing tumors.

    Journal: Carcinogenesis

    Article Title: Genetic deletion of sphingosine kinase 1 suppresses mouse breast tumor development in an HER2 transgenic model

    doi: 10.1093/carcin/bgx097

    Figure Lengend Snippet: SphK1 and CLDN2 levels are increased in HER2-positive breast cancers in humans. Tissue microarray containing breast cancer samples from 92 HER2-positive breast cancer patients and matched adjacent normal breast tissues (n = 34) are stained for SphK1 and CLDN2. Staining were scored based on the intensity (0–3) and proportion (0–3), and the total scores were used for the analysis. [(A) and (B)] SphK1 and CLDN2 expressions were compared with pathological features including grade, stage and hormone receptor status. [(C) and (D)] Representative image of high, moderate and low SphK1 and CLDN2 expressing tumors.

    Article Snippet: Partek pre-processes raw intensity files from microarray experiment using GCRMA’s background subtraction and uses quantile normalization as the normalization technique.

    Techniques: Microarray, Staining, Expressing